Isolation and characterization of mesenchymal stem cells from human amnion and decidua.
Objectives: The purpose of this study is to isolate a population of multipotent cells from human amnion and decidua, respectively.
Methods: Human placentas (gestational age, 30~42 weeks) were obtained after vaginal or cesarean deliveries. Amnions and deciduas were divided mechanically. The collected cells from the amnion and decidua were cultured. Cultured cells were immunophenotypically characterized. The adipogenic, osteogenic and neurogenic differentiation capacities were tested, and their growth kinetics were analyzed.